Abstract

Vipin Kumar Saini, Vikas Kumar, Disha Sharma, Saba Rana, Mohd Salman

ABSTRACT

Soil is the lifeblood of the earth and is considered one of the largest reservoirs of microbial diversity. Examining the diversity of soil microbial communities is important because they play an important role in maintaining soil health by recycling nutrients and building soil structure and humus. However, cultural studies cannot provide an accurate estimate of diversity and untapped resources. Therefore, there is a need to investigate microbial diversity using culture-independent methods. The field of metagenomics contributes to the study of the genomes of different soils with new resource potential in their own habitats. In this research, we collected and examined three different soil samples from agriculture, parks and roads. Additionally, soil samples were inoculated into food broth media to promote the growth of top bacteria. Additionally, genomic DNA was extracted from cultured cells and then PCR was used to amplify the 16S rRNA gene. 16S rRNA gene sequencing is used as a tool to identify disease types and help distinguish between closely related diseases. The 16S rRNA gene is a highly conserved transcription factor of all forms of DNA-based life, making it well-suited as a target for DNA sequencing in samples containing thousands of different species. Universal PCR primers can be designed to target the conserved region of 16S, allowing the amplification of genes from many different organisms from a single sample. In our study, we detected three different diseases in three different samples