Analysis of Macroscopic, Microscopic and TLC based Phytochemical characteristics of Melia azedarach L.
Shayma Saifi, Zehra Husaini, Fadak Zehra, Vandana Sharma
ABSTRACT
The study investigates the medicinal potential of Melia azedarach through a comprehensive analysis of its phytochemical composition and genetic characteristics. Phytochemical analysis of plant extracts revealed the presence of alkaloids, flavonoids, saponins, tannins, and carbohydrates, while extraction with methanol yielded a 13.5% dark green methanolic extract. Macroscopic and microscopic examinations provided insights into leaf morphology. Further confirmation of phytochemical constituents through qualitative analysis, thin layer chromatography, and UV-VIS spectrophotometry underscored the plant's diverse chemical profile. Successful DNA extraction using cTAB procedure for molecular genetic studies validated the authenticity of the genetic material. Overall, these findings emphasize the medicinal significance of Melia azedarach and suggest its potential for the development of novel therapeutic agents.
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