ISOLATION AND IDENTIFICATION OF MULTIDRUG-RESISTANT BACTERIA FROM PIG FEEDS AND FAECES
Bolaji A. S. and Taleat A. A. T.
ABSTRACT
A total of 15 bacteria were isolated from pig feeds and feace from two pig farms in Osun state. The isolated bacteria were identified by biochemical tests to include 3 strains of Pseudomonas aeruginosa, 2 strains of Corynrbacterium xerosis, 2 strains of Escherichia coli, 2 strains of Kiebsiella aerogenes, 2 stains of Providencia rettgeri and a strain of Micrococcus roseus, Staphylococcus epidermis, Citrobacter Freunlii and Proteus vulgaris. These organisms were subjected to antibiotics susceptibility tests and, 86.70% of the organisms showed multi-drug resistance. E. coli and Pseudomonas showed resistance to clinically important antibiotics. 73.30 % of all the bacteria isolates were susceptible to Gentamycin while between 40 to 50 % of the isolates were susceptible to all the antibiotic drugs used for the study. Persistence or unguided use of antibiotic drugs in livestock keeping could results in resistance of organisms to drugs thus reducing the efficacy of these life-saving drugs. Proximate analysis of pig feed samples indicates that brewery wastes (pig feed) had the highest amount of protein (24.95 % ± 0.06 ), crude fat (5.69 % ± 0.11), crude fibre (9.71 % ± 0.50), total ash content (7.87 % ± 0.21) and carbohydrate (42.85 % ± 3.51). However, there was no significant difference in the proximate compositions of all the pig feeds investigated.
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